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ansR [2019-08-15 14:16:42]
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ansR [2019-08-15 14:16:42]

transcriptional repressor ([SW|Xre family]) of the [gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|search|ansB ]operon
locus
BSU_23590
pI
4.81
mw
13.09 kDa
protein length
116 aa Sequence Blast
gene length
351 bp Sequence Blast
function
negative regulation of the [gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|search|ansB ]operon
product
transcriptional regulator ([SW|Xre family])
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.6|Utilization of asparagine/ aspartate]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

    • Gene

    Coordinates
    2,456,990 2,457,340

    Phenotypes of a mutant

  • the [gene|search|ansR ]mutation allows glutamate utilization of a ''[gene|56CBEBCFEF5CFB4A0175498338C7AF2F45EAA3E3|rocG] [gene|C36C8C9EEDCAD392D9BEC9728A1E0CBFF2F4E790|gudB]'' mutant [Pubmed|21219666]

    • The protein

    Protein family

  • [SW|Xre family]

    • [SW|Domains]

  • HTH cro/C1-type domain (aa 6-60) (according to UniProt)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|146947C974D62BCB282BA3F5B924B35695D75886|ansR]
    description
    [Pubmed|8478318]

    regulatory mechanism

  • [protein|146947C974D62BCB282BA3F5B924B35695D75886|AnsR]: negative autoregulation, undefined, in [regulon|146947C974D62BCB282BA3F5B924B35695D75886|AnsR regulon]

    • regulation

  • induced in the presence of asparagine ([protein|search|AnsR]) [Pubmed|11914346]
    • view in new tab

    Biological materials

    Mutant

  • GP1152 (del tet) available in [SW|Jörg Stülke]'s lab
  • BKE23590 ([gene|146947C974D62BCB282BA3F5B924B35695D75886|ansR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACTTCCGCTCCTTTT, downstream forward: _UP4_GAGGAACTGAGTTAATCTTT
  • BKK23590 ([gene|146947C974D62BCB282BA3F5B924B35695D75886|ansR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACTTCCGCTCCTTTT, downstream forward: _UP4_GAGGAACTGAGTTAATCTTT

    • Expression vectors

  • expression of native ''ansR'' in ''B. subtilis'': pGP873 (in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab

    • References

  • 8478318,11914346,21219666